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Back-Illuminated sCMOS with Large Pixels

Prime 95B

The Prime 95B was the first back-illuminated sCMOS camera, and offers extreme sensitivity thanks to 95% QE, large pixels and low noise. The Prime 95B also operates at high frame rates across a large sensor, consistently outperforming EMCCD cameras.

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Sensitivity

The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency.

By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging.

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Superior Background Quality

The Prime 95B features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern-free images with minimal pixel defects, delivering improved image quality in low light conditions.

 

Multiple Fields of View

The Prime 95B is available with an 18.8, 22 or 25 mm diagonal field of view. The larger format sensors are designed to increase throughput, maximize the amount of data captured and take full advantage of new, larger field of view microscopes.

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Advanced Features

The Prime 95B offers two data reduction technologies; PrimeLocate and Multi-ROI.

PrimeLocate can also be extended to offer Live Particle Tracking on the camera, giving early insight into the kinetics of moving molecules for single molecule tracking applications.

PrimeEnhance active denoising provides a real-time quantitative increase in signal to noise ratio by reducing the effects of photon shot noise at low light levels.

 

Advanced Triggering

Sequenced Multiple Acquisition Real Time (SMART) Streaming enables the Prime 95B to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.

The Prime 95B also supports up to four expose-out signals and has the capability for an effective global shutter.

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Family Specifications

Sensor Technology sCMOS
Quantum Efficiency 95%
Pixel Size 11 µm x 11 µm
Supported Interfaces PCIe and USB 3
Spectrum Capabilities UV (200-440 nm), Visible (400-700 nm), Near Infrared (700-1000 nm)
Read Noise (median) 1.6 e-

Model Finder

Product FOV (Diag. mm) MP FPS Pixel Size (μm) QE Read Noise Sensor Price Compare Options
Prime 95B
01-PRIME-95B-R-M-16-C
13.3 x 13.3 mm 1.4 80 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 144 BSI In Production Request Info Prime 95B
Prime 95B (Plus Features)
01-PRIME-95B-R-M-16-C-ENH-LOC
13.3 x 13.3 mm 1.4 80 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 144 BSI In Production Request Info Prime 95B
Prime 95B 22mm
01-PRIME-95B-22MM-R-M-16-C
15.5 x 15.5 mm 1.9 68 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 400 BSI In Production Request Info Prime 95B
Prime 95B 25mm
01-PRIME-95B-25MM-R-M-16-C
17.7 x 17.7 mm 2.5 60 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 400 BSI In Production Request Info Prime 95B

Media gallery

Running a Prime 95B Scientific CMOS camera in CellSens Software
Running a Prime 95B Scientific CMOS camera in CellSens Software

Resources & Support

Customer Story

Single Molecule FRET

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Customer Story

Single Molecule Imaging at University of Lund

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Customer Story

Single Molecule Imaging at University of York

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Customer Story

Single Molecule Imaging at University of Zürich

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Customer Story

Single Molecule Spectroscopy at Heidelberg University

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Customer Story

Single Molecule TIRF

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Customer Story

Single Molecule Tracking PALM

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Customer Story

Single-Molecule Biophysics

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Customer Story

Single-Molecule FRET

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Customer Story

smFRET, STORM and TIRF

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Below are assets shared by all models of this product family.

For model-specific assets, select an option in the Model Finder above.

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PVCAM SDK 3.10.1.1

Software Download

Version: PVCAM SDK 3.10.1.1

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PVCAM 3.10.0.3 (Linux)

Software Download

Version: 3.10.0.3

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PVCAM 3.8.0.6

Software Download

Version: 3.8.0.6

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3rd Party: Dotphoton Jetraw Raw Image Data Compression Tool

Software Download

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3rd Party: Intelligent Imaging Innovations (3i) Slidebook 6 Software

Software Download

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3rd Party: Leica Application Suite X (LAS X)

Software Download

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3rd Party: MathWorks MATLAB Image Acquisition Toolbox

Software Download

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3rd Party: Micro-Manager Open Source Microscopy Software

Software Download

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3rd Party: Molecular Devices MetaMorph

Software Download

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3rd Party: National Instruments LabVIEW System Design Software

Software Download

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Below are assets shared by all models of this product family.

For model-specific assets, select an option in the Model Finder above.

Prime 95B 22mm Datasheet

Datasheet

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Prime 95B 25mm Datasheet

Datasheet

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Prime 95B Customer Applications Handbook

Brochure

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Prime 95B Datasheet

Datasheet

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Prime 95B Family Manual

User Manual

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