Markets & Solutions  /  Scientific  /  Microscopy  /  Single Molecule

 

Scientific Imaging Solutions

Single Molecule

Fluorescence Microscopy

 
 

Classical biological techniques focus on describing the behavior of large ensembles of molecules. However, this doesn’t allow for the identification of subpopulations within the ensemble or show how these subpopulations behave under differing experimental conditions. The only way to investigate the behavior of single molecules or subpopulations is under strict experimental conditions, guaranteeing that each molecule is in the same state, one at a time.

Single-molecule fluorescence microscopy represents a subset of fluorescence microscopy that uses fluorescent tags to detect and analyze individual single molecules. This allows the activity of single molecules to be visualized with high signal-to-noise without disturbing the physiological conditions of the biological system.

Single molecule imaging is typically a very low-light technique so one of the main challenges is to collect as many of the emitted photons as possible to maximize signal to noise ratio so a highly sensitive camera is desired.

Single-molecule-01.png

 

Recommended Cameras for Single Molecule Microscopy

Resources from the Learning Center
View All
Application Note

Introduction To Single Molecule Microscopy

Read the Story
Application Note

Optical Trapping

Read the Story
Application Note

Labeling Proteins For Single Molecule Imaging

Read the Story
Customer Story

Interferometry and Lithium Mapping

Read the Story
Customer Story

Live Vesicle Trafficking

Read the Story
Customer Story

Simultaneous Multichannel SMLM

Read the Story
 

Request Info

Optin

By submitting you agree to Teledyne Vision Solutions' privacy policy and cookie policy.