Conventional light microscopy techniques are limited by the diffraction limit of light, preventing us from seeing anything smaller than approximately 200 nm in x and y and 500 nm in z.
Many molecules and structures of interest require a higher degree of resolution than this to visualize, so it was necessary to develop a technique to break the diffraction limit to see them. There are now a significant number of super-resolution techniques that break the diffraction limit of light such as localization based techniques (PALM/STORM), structural techniques (SIM/iSIM) and post-processing techniques (SRRF).
These techniques offer different advantages depending on the sample and application but typically require a scientific camera to have high sensitivity, high speed and high resolution with an additional advantage being granted by a large field of view.
Recommended Cameras for Super Resolution Microscopy
Prime 95B
Back-Illuminated sCMOS with Large Pixels
- Highly sensitive back-illuminated sCMOS for science
- 95% QE and low noise levels
- Large 11 µm pixels
Kinetix
High Speed, Large Sensor sCMOS
- Faster: 500 fps full-frame to capture dynamic samples
- Bigger: 29 mm, 10 MP sensor for high-content imaging
- More Sensitive: 96% QE and sub-electron read noise
Prime BSI Express
Compact, Powerful sCMOS for High-Speed Imaging
- Highly sensitive back-illuminated sCMOS for science
- 95% QE and ultra-low 1.0 e⁻ read noise
- 95 fps over USB 3.2
Resources from the Learning Center
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High Content Multiplex Fluorescence at Institute of Science and Technology Austria
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